Phytochemical Profile, in Vitro Antioxidant and Hemolytic Activities of Various Leaf Extract of Nymphaea Nouchali Linn: an in Vitro Study
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چکیده
Methods: The study was initiated by preliminary phytochemical screening to detect the presence of carbohydrates, flavonoids, alkaloids, saponins and tannins. Quantitative analysis of phenolic and flavonoids was carried out by Folins Ciocalteau reagent method. In vitro antioxidant activities were performed by DPPH free radical scavenging assay, Reducing power assay, Total antioxidant assay and Thiobarbituric acid assay. To check the toxicity of the extract on red blood cells, hemolytic activity was carried out by UV visible Spectrophotometric method. High Performance Liquid Chromatography (HPLC) analysis was performed to categorize type of phenolic compounds present in the bioactive extract.
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